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His-Tag mouse mAb(M1)

簡要描述:SourceMouseApplicationsWBReactivitySpeciesindependentDilutionWB:1:3000-5000Storage-20°C/1yearSpecificityTheantibodydetectsC-terminal

  • 產品型號:
  • 廠商性質:生產廠家
  • 更新時間:2025-09-21
  • 訪  問  量:238

詳細介紹

  • Source

    Mouse

  • Applications

    WB

  • Reactivity

    Species independent

  • Dilution

    WB: 1:3000-5000

  • Storage

    -20°C/1 year

  • Specificity

    The antibody detects C-terminal, internal, and N-terminal His-tag fusion proteins.

  • Source/Purification

    The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

  • Immunogen

    Synthetic peptide:HHHHHH conjugated to KLH.

  • Form

    PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

  • Clonality

    Monoclonal

  • Background

    The his-tag is a series of six to nine histidine residues generally fused to either the carboxy or amino terminus of a recombinant protein. The small size of the his-tag, compared with other common epitope tags, makes it less likely to obstruct the target protein’s structure or function and more suitable to use under denaturing conditions. The string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper. The binding to metal ions under specific buffer conditions, allows for the simple purification and detection of his-tagged proteins.

  • Concentration

    1 mg/ml

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